HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量

于宁; 何承辉; 邢建国

doi:10.11653/syfj2013160104

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HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量

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- HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量
- HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills
- 中国实验方剂学杂志 2013年19卷第16期页码：104-106
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区重大科技专项(201130105-3)
- DOI：10.11653/syfj2013160104
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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于宁, 何承辉, 邢建国. HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量[J]. 中国实验方剂学杂志, 2013,19(16):104-106.

YU Ning, HE Cheng-hui, XING Jian-guo. HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 104-106.
于宁, 何承辉, 邢建国. HPLC测定复方贯叶金丝桃滴丸中金丝桃苷的含量[J]. 中国实验方剂学杂志, 2013,19(16):104-106. DOI： 10.11653/syfj2013160104.

YU Ning, HE Cheng-hui, XING Jian-guo. HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 104-106. DOI： 10.11653/syfj2013160104.

摘要

目的: 建立复方贯叶金丝桃滴丸中主要有效成分金丝桃苷的含量测定方法。方法: 采用高效液相色谱法

COSMOSIL packed Column 5 C18-MS-Ⅱ(4.6 mm×250 mm

5 μm)色谱柱

流动相乙腈-水(0.5%冰醋酸和2%四氢呋喃)(13:87)

检测波长360 nm。结果: 金丝桃苷在10.8~54.0 mg·L-1峰面积与进样浓度具有良好线性关系(r=0.999 1)

平均回收率98.31% (n=6)

RSD 1.23%。结论: 该法准确、可行

可用于该滴丸的质量控制。

Abstract

Objective: To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills. Method: HPLC was used for the determination of hyperin in Compound Hypericum Perforatum. A COSMOSIL packed column 5 C18-MS-Ⅱ (4.6 mm×250 mm

5 μm) was used. The mobile phase was acetonitrile-water (0.5% glacial acetic acid and 2% tetrahydrofuran) (13:87). The detection wavelength was set at 360 nm. Result: The linear range for hyperin was 10.8-54.0 mg·L-1 (r=0.999 1). The average recovery was 98.31% (RSD 1.23%). Conclusion: The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively. It was sensitive

accurate and feasible.

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