Objective: To study the preventive effects of Haobie Yangyin Ruajian prescription (HBYYRJ) on rat hepatic fibrosis induced in rats by CCl4 and explore the effective mechanisms. Method: Rats were randomly divided into 7 groups:normal control group

model group

high

middle and low dose of HBYYRJ groups(8.20

2.59

0.82 g·kg-1)

compound Biejia Ruangan table group(0.54 g·kg-1)

colchicin group(1×10-4 g·kg-1). First CCl4 5 mL·kg-1 was given by sc

40% CCl4-peanut oil 3 mL·kg-1 was given by sc every three days from the second time to the twelfth time; 30% alcohol (1 mL) was ig given to model group and therapeutic groups every other day

drugs were given to the rats in therapeutic groups for six consecutive weeks

distilled water was given to the rats of normal and model groups. Histological observation of the liver tissue and collagen content were carried out. Blood serum was used for measuring the contents of alanine transaminase(ALT)

aspartate aminotransferase(AST)

hyaluronic acid(HA)

laminin(LN)

type III procollagen(PCIII)

type IV collagen(IVC)

albumin(ALB). The content of malondialdehyde (MDA) was detected by ultraviolet visible spectrophotometry. The content of hydroxyproline

collagen and albumin were detected in liver tissue. Result: The degeneration and necrosis

inflammatory cell infiltration

false lobular formation

and a large number of collagen fiber depositions were observed in liver tissue of the model group.The level of chronic hepatic damage was improved greatly and none of the false lobular was formed in the high dose of HBYYRJ. The contents of ALT

AST

HA and PCIII of the model group were significantly higher than that in normal group (P<0.01

P<0.05). The content of ALT

AST

HA and PCIII of HBYYRJ groups was significantly lower than that in model group (P<0.05). The content of MDA and collagen in liver tissue of HBYYRJ groups was lower than that in model group (P<0.01). The content of ALB of the high dose of HBYYRJ was significantly higher than that in model group (P<0.01). Conclusion: HBYYRJ has preventive effects on hepatic fibrosis in rats induced by CCl4.The mechanism might be associated with anti-lipid peroxidation

antiinflammation and restraining the compose of collagen protein.