Objective: To study effect of platelet rich plasma(PRP) combined with bone marrow mesenchymal stem cells(BMSCs) in treatment of rabbit femoral head necrosis. Method: Fifty rabbits were randomly divided into normal group

model group

PRP group (3%)

BMSCs group (1×106/mL) and combination group. The model of rabbit femoral head necrosis was made by operation. After 4 weeks of operation

the PRP group was implanted the PRP

the BMSCs group was implanted the BMSCs

the combination group implanted the PRP and BMSCs. Normal group was not treated. The rabbit femoral head necrosis were observed by HE trichrome coloration after 12 weeks of operation. The change of the trabeculae of bone

bone cells in the bone

fat cells were observed by light microscopy. Then PDGF protein in the rabbit femoral head was tested by Western blot. Result: Compared with the normal group

the trabecular of bone became thinner

decreased in bone cells

fat cells increased

empty osteocyte lacuna in the trabeculae were increased in model group (P<0.05). Compared with the model group

in all treatment groups trabecular bone became thicker

bone cells increased

fat cells decrease

empty osteocyte lacuna in the trabeculae were decreased and improved the expression of PDGF in the rabbit femoral head (P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration

improved the expression of PDGF and improve bone tissue recovery.