脾阳虚证失眠大鼠模型的建立和附子理中汤的干预效应

韦祎; 唐汉庆; 李克明; 李晓华; 朱晓莹

doi:10.11653/syfj2013160289

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脾阳虚证失眠大鼠模型的建立和附子理中汤的干预效应

更新时间：2024-01-04

- 脾阳虚证失眠大鼠模型的建立和附子理中汤的干预效应
- Establishment of Rat Spleen Yang Deficiency Syndrome Insomnia Model and Effect of Fuzi Lizhong Decoction on it
- 中国实验方剂学杂志 2013年19卷第16期页码：289-292
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- DOI：10.11653/syfj2013160289
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- 纸质出版日期：2013
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韦祎, 唐汉庆, 李克明, 等. 脾阳虚证失眠大鼠模型的建立和附子理中汤的干预效应[J]. 中国实验方剂学杂志, 2013,19(16):289-292.

WEI Yi, TANG Han-qing, LI Ke-ming, et al. Establishment of Rat Spleen Yang Deficiency Syndrome Insomnia Model and Effect of Fuzi Lizhong Decoction on it[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 289-292.
韦祎, 唐汉庆, 李克明, 等. 脾阳虚证失眠大鼠模型的建立和附子理中汤的干预效应[J]. 中国实验方剂学杂志, 2013,19(16):289-292. DOI： 10.11653/syfj2013160289.

WEI Yi, TANG Han-qing, LI Ke-ming, et al. Establishment of Rat Spleen Yang Deficiency Syndrome Insomnia Model and Effect of Fuzi Lizhong Decoction on it[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 289-292. DOI： 10.11653/syfj2013160289.

摘要

目的: 探讨附子理中汤对脾阳虚证失眠大鼠下丘脑增食素(orexin) mRNA表达及下丘脑-垂体-肾上腺轴(HPA axis)的影响及机制。方法: 100只Wistar大鼠随机分为正常组、模型组、附子理中汤低剂量组

中剂量组和高剂量组5组

每组20只。模型组按400 mg·kg-1ip对氯苯丙氨酸(PCPA)每天1次

连续2 d

建立脾阳虚证失眠模型。低剂量组、中剂量组、高剂量组分别以附子理中汤10

40 g·kg-1 ig

每日1次

连续7 d。疗程结束后对各组大鼠用ELISA法检测下丘脑5-羟色胺(5-HT)、促肾上腺皮质激素释放激素(CRH)及血浆促肾上腺皮质激素(ACTH)含量

RT-PCR法检测下丘脑orexin mRNA表达。结果: 与正常组相比

模型组orexin mRNA表达量显著升高(P<0.01)

5-HT含量显著降低(P<0.01);CRH

ACTH含量显著升高(P<0.05

P<0.01)。与模型组相比

附子理中汤高剂量组orexin mRNA表达量显著降低(P<0.01)

附子理中汤高、中剂量组5-HT含量显著升高(P<0.01

P<0.05);高剂量组CRH

ACTH含量显著降低(P<0.05

P<0.01)。结论: 附子理中汤下调orexin mRNA表达

抑制HPA 轴激活

降低应激反应性

可能是其治疗失眠的机制之一。

Abstract

Objective: To explore effect of Fuzi Lizhong decoction on the expression of hypothalamic orexin mRNA and hypcthalamopitutary-adrenol(HPA) axis in insomnia rats with spleen Yang deficiency syndrome and therapeutic mechanism. Method: Handred rats were randomly divided into control group

model group

low-dose group

middle-dose group

and high-dose group 5 groups. each group included 20 rats. Model rats were ip p-chlorophenylalanine(PCPA) according to 400 mg·kg-1

once a day for two days to establish insomnia model. low-dose group

middle-dose group

and high-dose group were ig given Fuzi Lizhong decoction respectively with 10

40 g·kg-1 based on model group for 7 days. Result: Compared with control group

expression of orexin mRNA of model group increased significantly (P<0.01).Compared with control group

content of 5-hydroxy tryptamine(5-HT) of model group reduced significantly (P<0.01).Compared with control group

content of corticotropin-releasing bormone(CRH) of model group increased (P<0.05). Compared with control group

content of adrenocorticotropic hormone(ACTH) of model group increased significantly(P<0.01). Compared with model group

expression of orexin mRNA of treatment group reduced significantly(P<0.01). Compared with model group

content of 5-HT of treatment group increased significantly (P<0.01)and content of 5-HT in middle-dose group increased (P<0.05). Compared with model group

content of CRH of treatment group reduced (P<0.05). Compared with model group

content of ACTH of treatment group reduced significantly (P<0.01). Conclusion: Fuzi Lizhong decoction can reduce expression of Orexin mRNA and so that inhibit HPA axis to reduce stress reaction

which perhaps is one of mechanisms for insomnia treatment.

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