Objective: To optimize laboratory extraction method of tissue proteins in Armeniacae Semen Amarum. Method: Tissue proteins in Armeniacae Semen Amarum were extracted by salting-out method

protein separation method

reverse micelles method

alkali extraction and acid precipitation method

alkaline method

the content of tissue protein was determined by Kjeldahl method

comparative analysis of proteins content and proteins extraction rate was adopted

kinds of extraction proteins was measured by application of a one-dimensional SDS-PAGE electrophoresis. Result: Extraction rates of tissue proteins using salting-out method

protein separation method

reverse micelles method

alkali extraction and acid precipitation method

alkaline method were 39.41%

25.63%

0.326%

40.02%

41.85%

respectively;protein contents were 75.667%

44.986%

5.372%

60.056%

51.379%

respectively;weights of protein powder were 8.6

9.1

0.823

9.8

6.259 g

while salting-out method showed the highest of proteins content and proteins extraction rate.SDS-PAGE electrophoresis pattern showed 4 electrophoretic bands by alkali extraction and acid precipitation method

protein separation method

reverse micelles method

salting-out method had 5 electrophoretic bands

alkaline method only 3

indicating that there was the largest number of protein species by salting-out method. Conclusion: Under laboratory conditions

salting-out method had the best effect on extracting proteins from tissue of Armeniacae Semen Amarum.