GC测定香榧假种皮挥发油中-蒎烯、-蒎烯和柠檬烯含量

薛海萍; 侯剑伟; 杨骏; 孙雯沁; 丁越; 张彤

doi:10.11653/syfj2013170057

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GC测定香榧假种皮挥发油中-蒎烯、-蒎烯和柠檬烯含量

更新时间：2024-01-04

- GC测定香榧假种皮挥发油中-蒎烯、-蒎烯和柠檬烯含量
- Simultaneous Determination of -Pinene,-Pinene and Limonene in Volatile oil from Aril of by GC
- 中国实验方剂学杂志 2013年19卷第17期页码：57-59
- 作者机构：
- 作者简介：
- 基金信息：
  
  教育部新世纪人才计划项目(NCET-10-0944);上海市创新团队及重点学科项目(J50302)
- DOI：10.11653/syfj2013170057
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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薛海萍, 侯剑伟, 杨骏, 等. GC测定香榧假种皮挥发油中-蒎烯、-蒎烯和柠檬烯含量[J]. 中国实验方剂学杂志, 2013,19(17):57-59.

XUE Hai-ping, HOU Jian-wei, YANG Jun, et al. Simultaneous Determination of -Pinene,-Pinene and Limonene in Volatile oil from Aril of by GC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 57-59.
薛海萍, 侯剑伟, 杨骏, 等. GC测定香榧假种皮挥发油中-蒎烯、-蒎烯和柠檬烯含量[J]. 中国实验方剂学杂志, 2013,19(17):57-59. DOI： 10.11653/syfj2013170057.

XUE Hai-ping, HOU Jian-wei, YANG Jun, et al. Simultaneous Determination of -Pinene,-Pinene and Limonene in Volatile oil from Aril of by GC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 57-59. DOI： 10.11653/syfj2013170057.

摘要

目的: 建立气相色谱法测定香榧假种皮挥发油中α-蒎烯、β-蒎烯和柠檬烯含量的方法。方法: 采用HP-1石英毛细管柱(0.25 μm×320.00 μm×29.9 m)

进样口温度230℃

FID检测器

检测器温度250℃

柱温程序升温(起始温度50℃

保持2 min

8℃·min-1升温至130℃

保持3 min)

载气N2

流速1.2 mL·min-1

进样方式为分流进样

分流比30:1

进样量1 μL

萘为内标物。结果: α-蒎烯、β-蒎烯和柠檬烯线性范围分别为0.070~2.230(r=1.000 0)

0.075~2.393(r=1.000 0)

0.070~2.240(r=0.999 9) g·L-1

平均回收率分别为103.0%(RSD 1.4%)

99.1%(RSD 1.2%)

97.7%(RSD 1.6%)。结论: 建立的测定方法简便快速、准确

可为香榧假种皮挥发油质量控制提供参考。

Abstract

Objective: To establish a method for simultaneous determination of α-pinene

β-pinene and limonene in volatile oil from aril of Torreya grandis by GC. Method: HP-1 quartz capillary column (0.25 μm×320.00 μm×29.9 m) was adopted

inlet temperature 230℃

flame ionization detector(FID) with temperature of 250℃

column temperature program (initial temperature 50℃

keeping 2 min

heated to 130℃ at 8℃·min-1

keeping 3 min)

N2 as carrier gas

flow rate 1.2 mL·min-1

injection mode was split injection with split ratio of 30: 1

injection volume 1 μL

naphthalene as an internal standard. Result: The linear ranges of α-pinene

β-pinene and limonene were 0.070-2.23(r=1.000 0)

0.075-2.393(r=1.000 0)

0.070-2.24 g·L-1 (r=0.999 9)

respectively;Average recoveries of them were 103.03%(RSD 1.42%)

99.17%(RSD 1.23%)

97.73%(RSD 1.63%)

respectively. Conclusion: This developed method was simple

quick and accurate

it could be used for quality control of volatile oil from aril of T. grandis.

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