Objective: To develop a HPLC method for simultaneous determination of magnoflorine

epiberberine

coptisine

jatronhizine

palmatine and berberine in Coptidis Rhizoma and Jinqi Jiangtang Tablets. Method: The chromatographic conditions were:Waters Symmetry ShieldTM RP18 column (4.6 mm×250 mm

5 μm)

acetonitrile (0.3% formic acid and 0.3% triethylamine) (A) and water (0.3% formic acid and 0.3% triethylamine) (B) as mobile phases for gradient elution;flow rate was set at 1.0 mL·min-1. The detection wavelength was set at 345 nm and 270 nm and the column temperature was maintained at 25℃. Result: The linear ranges of magnoflorine

epiberberine

coptisine

jatronhizine

palmatine and berberine were 0.012 06-0.241 2

0.038 24-0.764 8

0.088 02-1.760 4

0.016 55-0.331 0

0.036 08-0.721 6

0.189 41-3.788 2 μg (r≥0.999 8). The average recoveries were between 95%-105%. Conclusion: This is a specific

sensitive

repeatable and simple method for determining magnoflorine

epiberberine

coptisine

jatronhizine

palmatine and berberine in Coptidis Rhizoma and Jinqi Jiangtang Tablets. The method is available for the quality control of Jinqi Jiangtang Tablets.