Objective: To establish high performance liquid chromatographic fingerprint of the seeds of Sterculia lychnophora and compare the quality coherence of this drug commercially available in China in order to provide a reference for its quality evaluation. Method: The fingerprint analysis procedure of these seeds was developed by investigating the extraction and chromatographic conditions

the fingerprints of 8 batches of the seeds purchased from various places of China were determined with this procedure

and the data were analyzed with Chromatographic Fingerprint Similarity Evaluation System 2004A Version provided by Chinese Pharmacopoeia Commission. Result: The extraction and chromatographic conditions for HPLC fingerprint of the seeds of S. lychnophora were established

20 common peaks of this drug were identified

and similarities of these 8 batches were determined to be between 0.942 and 0.984. Conclusion: The HPLC fingerprint analysis procedure of the seeds of S. lychnophora has been successfully established for the first time. The quality of the seeds of S. lychnophora commercially available in China was demonstrated to be comparable by the established procedure.