UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量

何丹; 杨林; 秦少容; 张景勍

doi:10.11653/syfj2013170087

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UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量

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- UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量
- Determination of Content of Uridine,Guanosine and Adenosine in by UPLC
- 中国实验方剂学杂志 2013年19卷第17期页码：87-89
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2011BAI13B03)
- DOI：10.11653/syfj2013170087
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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何丹, 杨林, 秦少容, 等. UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量[J]. 中国实验方剂学杂志, 2013,19(17):87-89.

HE Dan, YANG Lin, QIN Shao-rong, et al. Determination of Content of Uridine,Guanosine and Adenosine in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 87-89.
何丹, 杨林, 秦少容, 等. UPLC法测定半夏提取物中尿苷、鸟苷和腺苷的含量[J]. 中国实验方剂学杂志, 2013,19(17):87-89. DOI： 10.11653/syfj2013170087.

HE Dan, YANG Lin, QIN Shao-rong, et al. Determination of Content of Uridine,Guanosine and Adenosine in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 87-89. DOI： 10.11653/syfj2013170087.

摘要

目的: 建立以超高效液相色谱(UPLC)法测定半夏提取物中尿苷、鸟苷和腺苷含量的方法。方法: 色谱柱ACQUITY UPLC BEH Cl8(2.1 mm×50 mm

1.7 μm)

流动相水-甲醇不同比例梯度洗脱

柱温30℃

流速0.5 mL·min-1

检测波长254 nm。结果: 尿苷在2.033~20.33 mg·L-1线性关系良好

回归方程为Y =16 788X-3 556(r=0.999 9)

平均回收率98.65%

RSD 2.12%;鸟苷在1.098~10.98 mg·L-1线性关系良好

回归方程为Y =25 378X-325(r=0.999 9)

平均回收率98.21%

RSD 2.32%;腺苷在0.201 2~2.012 mg·L-1线性关系良好

回归方程为Y=30 800X-59(r=0.999 9);平均回收率98.54%

RSD 1.91%。结论: 该方法准确、快速、可靠

能有效地测定半夏提取物中尿苷、鸟苷和腺苷的含量。

Abstract

Objective: To establish an ultra performance liquid chromatography(UPLC) method for detecting content of uridine

guanosine and adenosine in Pinellia ternata. Method: ACQUITY UPLC BEH Cl8(2.1 mm×50 mm

1.7 μm)was used

and the mobile phase was composed of methanol and water by gradient elution mode

column temperature was 3 0℃

at a flow rate of 0.5 mL·min-1 and detection wavelength of 254 nm. Result: The linear range of uridine was 2.033-20.33 mg·L-1. The regression equation was as follows:Y=16 788X-3 556(r=0.999 9). The linear range of guanosine was 1.098-10.98 mg·L-1. The regression equation was as follows:Y =25 378X-325(r=0.999 9). The linear range of adenosine was 0.201 2-2.012 mg·L-1. The regression equation was as follows:Y=30 800X-59(r=0.999 9). The average recovery was 98.65%(RSD 2.12%)

98.21%(RSD 2.38%) and 98.54%(RSD 1.91%) respectively. Conclusion: The method is accurate

rapid and reliable and can be used to determine the content of uridine

guanosine and adenosine in P. ternata.

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