Objective: To establish the quality standard of Huangbaizhili Granules. Method: The Coptis chinensis

Rhubarb charcoal and Pulsatilla chinensis were identified by TLC and the content of berberine hydrochloride was detected by HPLC. The separation was carried out on an Agilent Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile-0.3% phosphoric acid (32: 68). The detective wavelength was set at 348 nm. The column temperature was kept at 25℃. The flow rate was 1.0 mL·min-1 and the injection volume was 10 μL. Result: The C. chinensis

R. charcoal and P. chinensis could be identified by TLC and the calibration curve of berberine hydrochloride showed a good linearity in the range of 0.008 8-0.11 g·L-1(r=0.999 1). The average recovery was 98.58% with RSD 1.05%. Conclusion: This method to identify three main ingredients in Huangbaizhili Granules by TLC is simple and has a great repeatability. The negative control sample has no interference. The method to determine the content of berberine hydrochlorid in Huangbaizhili Granules is rapid

accurate and the results have a good repeatability and recovery. The methods can be used for the quality of the Huangbaizhili Granules.