HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量

代丽萍; 谢小龙; 鲁艳清; 王娟

doi:10.11653/syfj2013170127

您当前的位置：

首页 >

文章列表页 >

HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量

更新时间：2024-01-04

- HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量
- Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC
- 中国实验方剂学杂志 2013年19卷第17期页码：127-129
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省教育厅重点研究项目(12A360001);河南中医学院博士基金项目(BSJJ2010-02)
- DOI：10.11653/syfj2013170127
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
代丽萍, 谢小龙, 鲁艳清, 等. HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量[J]. 中国实验方剂学杂志, 2013,19(17):127-129.

DAI Li-ping, XIE Xiao-long, LU Yan-qing, et al. Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 127-129.
代丽萍, 谢小龙, 鲁艳清, 等. HPLC测定丹芍方提取物中丹皮酚、芍药苷的含量[J]. 中国实验方剂学杂志, 2013,19(17):127-129. DOI： 10.11653/syfj2013170127.

DAI Li-ping, XIE Xiao-long, LU Yan-qing, et al. Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 127-129. DOI： 10.11653/syfj2013170127.

摘要

目的: 建立HPLC同时测定丹芍方提取物中丹皮酚、芍药苷含量的方法。方法: 采用ODSHYPERSIL色谱柱(4.6 mm×250 mm

5 μm)

乙腈-水梯度洗脱

柱温32℃

流速0.8 mL·min-1

检测波长230

274 nm。结果: 丹皮酚、芍药苷分别在0~0.202 1

0~27.960 0 μg线性关系良好

平均回收率分别为97.40%

101.35%

RSD分别为1.18%

0.48%。结论: 3批样品测定结果表明

建立的方法简便、准确、重复性好

可以用于丹芍方提取物中丹皮酚、芍药苷的含量测定。

Abstract

Objective: To establish a method of HPLC for quantitative determination of paeonol and paeoniflorin in the extraction of Danshao Formula. Method: The separation was performed on the chromatographic column of ODS HYPERSIL (4.6 mm×250 mm

5 μm)

and the mobile phase in the gradient elution program was acetonitrile-water. The column temperature was maintained at 32℃. The flow rate was 0.8 mL·min-1

and the detection wavelength was set at 274 nm and 230 nm respectively. Result: Paeonol and paeoniflorin showed good linear relationship in the range of 0-0.202 1

0-27.960 0 μg. The average recovery was 97.40% (RSD 1.18%)

101.35% (RSD 0.48%) respectively. Conclusion: The method is easy and accurate with higher repeatability

which can be used in the quality control of paeonol and paeoniflorin in the extraction of Danshao formula.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC测定不同厂家桂枝茯苓丸中丹皮酚及芍药苷

毛冬青饮片指纹图谱及定量分析

铁皮石斛与霍山石斛中甘露糖、葡萄糖及柚皮素的含量比较

荆银颗粒质量标准的实验研究