Objective: To establish the quality standard for Evodiae juice

an adjuvant material of processing. Method: The Evodiae juice was identified by TLC. The content of evodin

evodiamine and rutaecarpine in the Evodiae juice was determined by HPLC simultaneously. The column of Agilent C18 (4.6 mm×250 mm

5 μm) was used. The mobile phase consisted of acetonitrile-water-tetrahydrofuran-glacial acetic acid (40: 60: 1: 0.2). The flow rate was 1.0 mL·min-1. The wavelength was set at 225 nm for evodin and evodiamine

343 nm for rutaecarpine. Result: TLC pots were clear with strong specificity

good repeatability and easy identification. While the linear range of evodin

evodiamine and rutaecarpine were 0.12-8.10 μg (r=0.999 9)

0.028-1.80 μg (r=0.999 8)

0.012-0.75 μg (r=0.999 9). The average recoveries of three compounds were 101.49% with RSD 2.21%

102.65% with RSD 1.51% and 100.47% with RSD 3.42%(n=6) respectively. Conclusion: The method is convenient

accurate and reproducible. It can be used for the quality control of Evodiae juice

the adjuvant material.