HPLC测定醒脑静注射液中莪术二酮及吉马酮的含量

杨立新; 刘薇薇; 甘国锋; 周福康; 何希荣; 李春

doi:10.11653/syfj2013170136

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HPLC测定醒脑静注射液中莪术二酮及吉马酮的含量

更新时间：2024-01-04

- HPLC测定醒脑静注射液中莪术二酮及吉马酮的含量
- Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography
- 中国实验方剂学杂志 2013年19卷第17期页码：136-139
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- DOI：10.11653/syfj2013170136
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- 纸质出版日期：2013
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杨立新, 刘薇薇, 甘国锋, 等. HPLC测定醒脑静注射液中莪术二酮及吉马酮的含量[J]. 中国实验方剂学杂志, 2013,19(17):136-139.

YANG Li-xin, LIU Wei-wei, GAN Guo-feng, et al. Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 136-139.
杨立新, 刘薇薇, 甘国锋, 等. HPLC测定醒脑静注射液中莪术二酮及吉马酮的含量[J]. 中国实验方剂学杂志, 2013,19(17):136-139. DOI： 10.11653/syfj2013170136.

YANG Li-xin, LIU Wei-wei, GAN Guo-feng, et al. Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 136-139. DOI： 10.11653/syfj2013170136.

摘要

目的: 建立醒脑静注射液中莪术二酮及吉马酮的含量测定方法。方法: 采用HPLC对醒脑静注射液中莪术二酮和吉马酮进行含量测定。Waters symmetry C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(60:40)

检测波长210 nm

柱温35℃

流速1.0 mL·min-1。结果: 莪术二酮在0.072 9~1.98 μg(r=0.999 9)

吉马酮在0.023 2~0.58 μg(r=0.999 9)呈良好的线性关系。莪术二酮和吉马酮的平均回收率分别为99.47%

100.2%

RSD分别为2.73%

2.94%。结论: 所建立的方法简便

重复性好

可用于醒脑静注射液的质量控制。

Abstract

Objective: To establish a method for simultaneous quantitative determination of curdione and germacrone in Xingnaojing injection. Method: HPLC was used to determine the content of curdione and germacrone in Xingnaojing injection. The analysis was performed on an Waters symmetry C18 column. The mobile phase was acetonitrile-water(60: 40)

the monitoring wavelength was 210 nm

the column temperature was 35℃ and the flow rate was 1.0 mL·min-1. Result: The linear ranges of curdione and germacrone were 0.072 9-1.98 μg (r=0.999 9) and 0.023 2-0.58 μg (r=0.999 9) respectively. The average recovery of curdione and germacrone were 99.47% (RSD 2.73%) and 100.2%(RSD 2.94%) respectively

which showed the method was reliable. Conclusion: The method was simple and repeatable

and can be used for the quality control of Xingnaojing injection.

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