Objective: To determine the content and analyze the dynamic changes of the total flavonoids and five flavonoid components of vitexin-glucoside

vitexin-rhamnoside

vitexin

rutin

hyperoside of different months in hawthorn leaves from Chengde

and then determine the optimal harvest time. Method: The content of the total flavonoids in hawthorn leaves was determined by UV spectrophotometry

and the five flavonoid components were determined by high performance liquid chromatography (HPLC) to analyze the dynamic changes of the content in different months. An Agilent ZORBAX SB-C18 column (4.6 mm×250 mm

5 μm) was used with the mobile phase of 0.1% formic acid aqueous solution-acetonitrile-tetrahydrofuran at 30℃. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 340 nm. Injection volume was 10 μL. Result: The content of the total flavonoids and vitexin-rhamnoside

vitexin-glucoside

hyperoside in hawthorn leaves were the highest in June. The content of vitexin and rutin was the highest in July. Conclusion: It is suggested that the best harvest time of hawthorn leaves for extracting flavonoid components is in June.