Objective: To determine pharmacokinetic parameters of ferulic acid after oral administration by blood microdialysis technique. Method: Days stability of probe recovery and effects of perfusion rate

the concentration of ferulic acid

temperature on probe recovery were investigated by concentration difference method (incremental and reduction method);Zero-net flux method was adopted to determine in vitro recovery of ferulic acid

result of this method was compared with concentration difference method.In vivo pharmacokinetic study was applied by blood microdialysis method

the content of ferulic acid was determined by HPLC. Result: Days stability of probe recovery was good;In vitro recovery had no correlation with the concentration of ferulic acid

but presented exponential negative correlation with perfusion rate and positive correlation with temperature;The content of ferulic acid and recovery in dialysis medium could be accurately determined by zero-net flux method.Recovery of incremental method

reduction method and zero-net flux method was the same.In vivo average recovery was (28.69±1.95)% with flow rate of 2.5 μL·min-1

Cmax of ferulic acid was reached at 9.33 min and t1/2β was 12.52 min after oral administration. Conclusion: Microdialysis sampling technique could be used for pharmacokinetic study of ferulic acid and reduction method could be used to determine recovery of ferulic acid in in vivo microdialysis study.