蟾酥提取物中3种蟾蜍甾烯类成分比格犬体内药代动力学

刘冬; 何秀峰; 杜守颖; 王爱国; 代春红; 顾雪竹; 赵立敏

doi:10.11653/syfj2013170188

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蟾酥提取物中3种蟾蜍甾烯类成分比格犬体内药代动力学

更新时间：2024-01-04

- 蟾酥提取物中3种蟾蜍甾烯类成分比格犬体内药代动力学
- Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs
- 中国实验方剂学杂志 2013年19卷第17期页码：188-192
- 作者机构：
- 作者简介：
- 基金信息：
  
  中药生产技术及过程控制技术标准平台(2009ZX09308-003)
- DOI：10.11653/syfj2013170188
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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刘冬, 何秀峰, 杜守颖, 等. 蟾酥提取物中3种蟾蜍甾烯类成分比格犬体内药代动力学[J]. 中国实验方剂学杂志, 2013,19(17):188-192.

LIU Dong, HE Xiu-feng, DU Shou-ying, et al. Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 188-192.
刘冬, 何秀峰, 杜守颖, 等. 蟾酥提取物中3种蟾蜍甾烯类成分比格犬体内药代动力学[J]. 中国实验方剂学杂志, 2013,19(17):188-192. DOI： 10.11653/syfj2013170188.

LIU Dong, HE Xiu-feng, DU Shou-ying, et al. Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 188-192. DOI： 10.11653/syfj2013170188.

摘要

目的: 建立犬血浆中蟾酥提取物中3种蟾蜍甾烯类化合物蟾毒灵、华蟾酥毒基及酯蟾毒配基的分析方法

并用于其在犬体内的药代动力学研究。方法: 比格犬按0.18 mg·kg-1由股静脉注射蟾酥提取物后分别于2

90 min股静脉取血

采用乙腈沉蛋白与固相微萃取相结合方法进行血浆样品预处理

以HPLC方法测定犬血浆中蟾毒灵、华蟾酥毒基及酯蟾毒配基的浓度

以Kinetica软件拟合药动学参数。结果: 建立的SPE-HPLC方法

蟾毒灵、华蟾酥毒基及酯蟾毒配基均得到很好的分离

重复性、精密度、线性关系良好

达到体内分析要求;经非房室模型拟合

得到了蟾毒灵、华蟾酥毒基及酯蟾毒配基在犬体内主要药动学参数。结论: 建立的蟾毒灵、华蟾酥毒基及酯蟾毒配基比格犬血药浓度SPE-HPLC方法及所获得的药动学参数

可为中药蟾酥提取物的药代动力学研究提供参考。

Abstract

Objective: To establish a HPLC method for the assay of bufalin(BL)

cinobufagin(CBG)

resibufogenin(RBG) and study the pharmacokinetics of BL

CBG

RBG in dogs plasma. Method: The sample blood from dogs were injected Chan Su extract by 0.18 mg·kg-1 at 2

90 min. Protein precipitation with acetonitrile and solid-phase extraction were applied to purify plasma samples and used HPLC to determine the concentration of BL

CBG

RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result: In this SPE-HPLC method

the separation

precision and accuracy of BL

CBG

RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90%

intra and inter-day precision RSD were all less than 10.0%(n=5). The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion: The SPE-HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL

CBG and RBG.

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