Objective: To investigate the protective effect of Danhong injection on cerebral ischemia-reperfusion injury in rats and hypoxic injury in Neuro-2A cell. Method: ①Adult male Wistar rats were randomly divided into 6 groups as following:model group

sham group

Danhong injection low

middle and high dose(0.9

1.8

3.6 mL·kg-1)groups

and the positive control (edaravone

6 mg·kg-1) group. The model of focal cerebral ischemia-reperfusion injury was reproduced by middle cerebral artery occlusion (MCAO) performed with thread inserted for 60 minutes and then reperfusion 72 h

Danhong injection was administered intraperitoneally to the animals 30 min before operation and immediately at the onset of reperfusion

and then once a day for three days. Scoring method of ischemia for 72 h was used to evaluate the abnormal behaviour by nerve injury. The content of S100B protein and nerve specific enolase (NSE) in brain tissue were determined by enzyme linked immunoassay(ELISA).②Neuro-2A cells injury were reproduced by oxygen and glucose deprivation (OGD). Incubating with Danhong injection(1.25

2.5

5 μL·mL-1)

the neuroprotective effect indexes were investigated as the cell proliferation activity

LDH release

and ROS detection. Result: ①Compared with sham group

the contents of S100B and NSE were significantly increased in brain tissue (P<0.01). Compared with model operation group

Danhong injection 0.9

1.8

3.6 mL·kg-1 ip could improve the nerve defect symptoms of model rats at 72 h to some extent(P<0.05

P<0.01

P<0.05)

and decreased the content of NSE significantly(P<0.05). Danhong injection 0.9

1.8 mL·kg-1 ip could markedly decrease the content of S100B protein(P<0.05

P<0.01).②Compared with control group

the viability of Neuro-2A cell injury induced by OGD decreased significantly

the release of LDH and the level of ROS significantly increase(P<0.01)

1.25

2.5

5 mL·L-1 Danhong injection could improve hypoxic injury Neuro-2A cell proliferative activity

inhibit the level of ROS increase in cells(P<0.01)and 2.5

5 mL·L-1 Danhong injection could reduce the LDH release of Neuro-2A cell(P<0.05

P<0.01). Conclusion: Danhong injection protect against cerebral the hypoxic-ischemic injury.