Objective: To investigate the effect of Yinhuang mixture on the expression of multidrug resistance-associated protein 2 (MRP2) and bile salt export pump (BSEP) in rats with intrahepatic cholestasis of pregnancy induced by estrogen and progesterone. Method: The animal model of intrahepatic cholestasis of pregnancy was induced by 17-α-ethinylestradiol and progesterone and these rats were randomly divided into four groups

including normal group

model group

low dosage and high dosage of Yinhuang mixture groups

either saline or low and high dosage of Yinhuang mixture were given. The serum levels of total bile acid (TBA) total bilirubin (TBil) alanine transarninase(ALT) aspartate aminotransferase

(AST)were examined

and the expression of MRP2 and BSEP were detected by real-time RT-PCR and Western blotting analysis. Result: Compared with the model group

the serum levels of TBA and TBil were remarkably decreased in low dosage and high dosage of Yinhuang mixture groups

in addition

the expression of MRP2 and BSEP were significantly increased in these groups. Conclusion: The therapeutic effects of Yinhuang mixture on intrahepatic cholestasis of pregnancy may upregulate the expression of MRP2 and BSEP.