Objective: To screen anticonvulsive activity of compounds isolated from Beauveria bassiana. Method: Two anticonvulsive models indued by nikethamide (750 mg·kg-1

sc)and isoniazid(300 mg·kg-1

sc) were used to test the three compounds(β-sitosterol; ergosta-6

22-diene-3β

5α

8α-triol; beauvericin) isolated from B. bassiana.After 3 days of subcutaneous injection of the three compounds

nikethamide (750 mg·kg-1

sc)and isoniazid(300 mg·kg-1

sc)were injected after 30 minutes of the end administration. Result: Compound Ⅰ(β-sitosterol)

had no significantly effect on the two models;high-dose of compoundⅡ(ergosta-6

22-diene-3β

5α

8α-triol

250 mg·kg-1) prolonged the latent time of convulsions induced by nikethamide while low-level (beauvericin 125 mg·kg-1) increased the death time induced by isoniazid;low-level of compoundⅢ (beauvericin

125 mg·kg-1)prolonged the latent time of convulsions induced by nikethamide and high-level (250 mg·kg-1)prolonged the death time induced by isoniazid. Conclusion: CompoundⅢ(beauvericin) has anticovulsant activity

can be as a quality control index component of B. bassiana.