Objective: To observe the inhibitory effect of serum containing Huazhuo Jiedu recipe on oxidative stress in hepatic stellate cell(HSC)-T6 cells. Method: HSC-T6s were divided into control group

model group and serum containing Huazhuo Jiedu recipe group. HSC-T6s in control group were only incubated with high glucose DMEM

HSC-T6s in model group were treated with 0.1 mmol·L-1 H2O2. The methods of thiobarbituric acid

xanthine oxidase and digestion were respectively applied to determine the levels of malondialdehyde(MDA)

superoxide dismutase(SOD)and hydroxyproline(HyP)of culture mediums.The mRNA expression of collagen(COL) I and COLⅢ was determined by RT-PCR and the protein of COL I and COLⅢ was determined by Western blot. Result: Compared with control group

the level of SOD and GSH-Px decreased.the level of MDA and GSH increased significantly in model group(P<0.05). The activity of SOD and GSH-Px increased and the level of MDA and GSH decreased significantly in serum containing Huazhuo Jiedu recipe groups(P<0.05)

especially in the high does and middle does group.The mRNA and the protein expression of COL I and COLⅢ increased in model group

decreased in Huazhuo Jiedu recipe groups(P<0.05). Conclusion: The serum containing Huazhuo Jiedu recipe can dilute the level of oxidation

depress the content of collegen of HSC and lessen fibrosjs.