Objective: To refine and preliminarily analysis chemical structure of polysaccharides from Caulis Polygoni Multiflori. Method: Through degreasing

water extraction

classification alcohol precipitation

bleaching

dialysis

crude polysaccharides was prepared

then separated and purified by DEAE 52-cellulose anion exchange column chromatography and Sephacryl S-300 gel permeation chromatography.Physical and chemical properties of polysaccharides were analyzed by chemical methods

purity

relative molecular mass

monosaccharide composition and preliminary chemical structure of polysaccharides were analyzed by high performance gel permeation chromatography(HPGPC)

gas chromatography(GC)

ultraviolet spectrometry(UV)

infared spectroscopy(IR)

et al. Result: Six homogeneous polysaccharides(SWTPA-1

SWTPB-1

SWTPB-2

SWTPB-3

SWTPC-1

SWTPC-2) were obtained

their relative molecular mass were 9 162

56 314

44 502

37 278

40 558

61 000

respectively.All these polysaccharides did not contain macromolecular proteins

peptides

nucleic acids

starches

phenols

but contained IR characteristic absorption peaks of carbohydrates.SWTPA-1

SWTPC-1 and SWTPC-2 were neutral polysaccharide;SWTPB-1

SWTPB-2 and SWTPB-3 may be acidic polysaccharides;SWTPA-1

SWTPB-1

SWTPB-3

SWTPC-1 and SWTPC-2 were pyranose and contained β glycosidic bond;SWTPB-2 was pyranose and contained α and β glycosidic bonds;SWTPB-2

SWTPB-3

SWTPC-1 and SWTPC-2 also contained furanose ring. Conclusion: Six homogeneous polysaccharides were first separated and purified from Caulis Polygoni Multiflori and their structures were preliminary analyzed

Sephacryl S-300 gel permeation chromatography could be adpoted to separate and purify of polysaccharides

sugar alcohol acetate derivatization method could better achieve polysaccharides from Caulis Polygoni Multiflori hydrolyzate derivatization.