LIU Rong, WEI Zheng, YIN Ling, et al. Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 23-26.
LIU Rong, WEI Zheng, YIN Ling, et al. Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 23-26. DOI: 10.11653/syfj2013180023.
Objective: To optimize extraction process of polysaccharides from Citri Reticulatae Pericarpium by central composite design-response surface methodology. Method: Based on single-factor tests
with solid-liquid ratio
extraction temperature
extraction time as independent variables and yield of polysaccharides from Citri Reticulatae Pericarpium as dependent variable
multiple linear regression and binomial fitting was adopted by central composite design model
and determine optimum extraction technology. Result: The best extraction process was as following:ratio of solid to liquid 1: 49
extraction temperature 92 ℃
reflux extraction time 150 min.Yield of polysaccharides from Citri Reticulatae Pericarpium was up to 14.77%
deviation of the predictive value and the measured value was 0.61%. Conclusion: This optimized extraction process was simple and reliable
it could laid a certain foundation for development and utilization of polysaccharides from Citri Reticulatae Pericarpium.