Objective: Changing regularity of chlorogenic acid and rutin content were detected from Ilex hainanensis gathered in different periods by HPLC in order to decide the best gathering time. Method: Six samples of I. hainanensis were collected from July 2011 to May 2012 at every two months. RP-HPLC was used to determine the content of chlorogenie acid and rutin in different periods of I. hainanensis with the chromatographic conditions of Phenomenex Gemini C18 column (4.6 mm×250 mm

5 μm)

detected at the wavelength of 330 nm

gradient elution of acetonitrile-0.1% phosphoric acid solution as the mobile phase

column temperature of 30 ℃

the flow rate was 1 mL·min-1. Result: The linearity of chlorogenic acid and Rutin were good in the range of 0.10-2.00 μg and 0.25-5.00 μg

regress equation were Y=4 027X+5.262(r=1.000 0) and Y=1 258X-1.406(r=1.000 0)

the average recovery was 97.18% and 99.36%

RSDs were 1.27%

1.91%. The content of Chlorogenic acid and Rutin were higher in I. hainanensis collected in January

and the content of Chlorogenie acid and Rutin were lower in I. hainanensis collected in July. So the collection time could be set at January. Conclusion: The determination method of chlorogenic acid and rutin in I. hainanensis is good at resolution and reproducibility

quickly analysis

accurate and stable

which can be used for the quality control of I. hainanensis. There are some changing regulations of chlorogenic acid and rutin in llex hainanensis during its growing development period. The content of chlorogenic acid and rutin will help us to decide the best gathering time for I. hainanensis.