Objective: To investigate the chemical constituents of Ampelopsis japonica. Method: Multi-chromatographic methods including Silica gel column chromatography and Sephadex LH-20 gel column chromatography were employed for the isolation and purification. The structures were identified on the basis of chemical evidence and spectral data. Result: Fifteen compounds were isolated and identified as lupeol (1)

β-sitosterol (2)

physeion (3)

β-sitosterol linoleate (4)

paeonol (5)

4-p-menthane-1

8-diol (6)

vanillin (7)

α-tocopherol (8)

α-tocopheryl quinone (9)

α-methyl-pyrrole-ketone (10)

botcinins D (11)

5α

8α-epidioxyergosta-6

22-dien-3β-ol (12)

4-keto pinoresinol (13)

bungein A (14)

lasiodiplodin (15). Conclusion: Compounds 4-15 are obtained from this plant for the first time.