Objective: To study chemical constituents from endophytic fungus FL10 of Ficus carica. Method: The compounds were isolated by column chromatography and identified on the basis of physic-chemical constants and spectral analysis. Result: Six indolyl diketopiperazines alkaloid were obtained and elucidated as verruculogen (1)

cyclotryprostatins B (2). fumitremorgin C (3)

cyclotryprostatin A (4)

tryprostatin A (5)

tryprostatin B (6). Conclusion: Six indolyl diketopiperazine alkaloids were isolated from endophyic fungus of F. carica for the first time. The present study revealed that endophytic fungi Aspergillus tamarii from F. carica are the sources for the production of indolyl diketopiperazine alkaloids.