Objective: The active components with function of eliminating fire and detoxication from Sanhuang Xiexin tang were annalyzed to provide scientific data for the discovery of active components and the establishment of quality evaluation methods. Method: The active components were determined by reversed phase high-performance liquid chromatography (RP-HPLC) compatibility. Result: The active components with function of eliminating fire and detoxication were Alkaloids-Flavonoids-Anthraquinones.Therwere 16 kinds of active components. The content of hydrochloride gatrorrhizine

hydrochloride epiberberine

hydrochloride coptisine

hydrochloride palmatine and hydrochloride berberine

baicalin

wogonoside

baicalein

wogonin

sennoside A and sennoside B

aloe-emodin

rhein

emodin

chrysophanol and physcion respectively was 1.80%

5.40%

7.70%

4.50%

18.80%

47.80%

9.40%

3.60%

1.10%

0.42%

3.54%

0.07%

0.11%

0.37%

0.11%

0.03%. Conclusion: The active compounds with function of eliminating fire and detoxication from Sanhuang Xiexin tang is 104.75%(mg·g-1)

and the 16 kinds ratio was 56: 163: 232: 136: 571: 1448: 283: 110: 32: 107: 13: 2: 3: 11: 3: 1.