HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量

任欣欣; 李兴华; 叶莎; 马小亚; 雷建林; 李万静; 段宗明

doi:10.11653/syfj2013180131

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HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量

更新时间：2024-01-04

- HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量
- Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC
- 中国实验方剂学杂志 2013年19卷第18期页码：131-134
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  陕西省科技攻关项目
- DOI：10.11653/syfj2013180131
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- 纸质出版日期：2013
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任欣欣, 李兴华, 叶莎, 等. HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量[J]. 中国实验方剂学杂志, 2013,19(18):131-134.

REN Xin-xin, LI Xing-hua, YE Sha, et al. Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 131-134.
任欣欣, 李兴华, 叶莎, 等. HPLC测定复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量[J]. 中国实验方剂学杂志, 2013,19(18):131-134. DOI： 10.11653/syfj2013180131.

REN Xin-xin, LI Xing-hua, YE Sha, et al. Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 131-134. DOI： 10.11653/syfj2013180131.

摘要

目的: 建立复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量测定方法。方法: 采用HPLC

流动相甲醇-0.5%的三乙胺(70:30)

Aglient TC-C18色谱柱(4.6 mm×250 mm

5 μm)

检测波长235 nm

流速 1.0 mL·min-1

柱温室温。结果: 乌头碱的进样量在0.080 8~0.808 0 μg具有良好的线性关系(r=0.999 6)

平均回收率98.40%

RSD 2.84%;新乌头碱的进样量在0.866~4.330 μg具有良好的线性关系(r=0.999 7)

平均回收率98.04%

RSD 2.05%;次乌头碱的进样量在0.089~0.890 μg具有良好的线性关系(r=0.999 8)

平均回收率98.71%

RSD 2.34%。结论: 方法操作简便、灵敏度高、结果准确

可作为复脉灵胶囊的含量测定方法。

Abstract

Objective: To establish a method for the determination of aconitine

mesaconitine and hypaconitine in Fumailing Capsules. Method: The aconitine

mesaconitine and hypaconitine in the capsules was determined by HPLC on the Aglient TC-C18column (4.6 mm×250 mm

5 μm) at room temperature. The mobile phase was consisted of methanol-0.05% triethylamine (70: 30). The flow rate was 1.0 mL·min-1 minand the detection wavelength was set at 235 nm. Result: The content of aconitine had a good linearity in the range of 0.080 8-0.808 0 μg (r=0.999 6)

the average recovery was 98.40%

RSD was 2.84%. The content of mesaconitine had a good linearity in the range of 0.866-4.330 μg (r=0.999 7)

the average recovery was 98.04% and RSD was 2.05%. The content of hypaconitine had a good linearity in the range of 0.089-0.890 μg (r=0.999 8)

the average recovery was 98.71%

and RSD was 2.34%; Conclusion: This method was simple

reproducible and accurate

and it can be used for determination of Fumailing Capsules.

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