Objective: The aim is to establish the determination method of saccharide in Pareonia albiflota extracts. Method: The volume ratio of sulfuric acid and water

the volume of color liquid

100 ℃ water bath time

and ice bath time were studied by single-factor experiment. Result: The optimum experimental conditions were as following: 5: 1 of the volume ratio of sulfuric acid and water

5 mL color liquid

100 ℃ water bath for 50 min

and ice bath for 15 min. In the optimum experimental conditions

the standard solutions of glucose possessed good linear with absorbance when its concentration was 0.019-0.189 mg. The linear equation was Y=7.488X-0.000 2(R2=0.999 5). The recovery ratio of three degrees was 102%

102.15%

95.86%

the RSD was 2.08%

1.51%

2.24%. The RSD of six samples was 2.4%. Conclusion: The method is simple

accurate and reliable. It can be used for the quality control of traditional Chinese medicine.