Objective: To observe the effect of compound Fufangteng mixture medicated serum on bone marrow hematopoietic stem cells (BMSCs) Notch signal pathway in rats. Method: The whole bone marrow adherent method was used to isolate

culture and purify the BMSCs in vitro.A total of 30 rats was randomly divided into 2 groups

15 each group

and compound Fufangteng mixture 2 g·kg-1·d-1 was given by gavage to the rats as drug intervention group

while physiological saline 2 mL·kg-1·d-1 was given as negative control group. The 10% medicine serum and negative control serum were intervene to BMSCs. Then RT-PCR was used to detect the mRNA expression of Notch1

Jagged1 and Hes1 after intervention. And the immunofluorescence cytochemical method was used to detect the protein expression of Notch1 and Jagged1 after the intervention. Result: RT-PCR results showed that the mRNA expression of Notch1

Jagged1 and Hes1 in drug intervention group was higher than negative control group(P<0.05). Immunofluorescence cytochemical method indicated that the protein expression of Notch1 and Jagged1 in drug intervention group was higher than that in negative control group(P<0.05). Conclusion: Compound Fufangteng mixture containing serum can upregulate key genes of Notch signal pathway and protein expression

and activate Notch signal pathway.