In this paper

we summarized the development of in-vitro release methods

including typical in vitro release method

dialysis bags or tube diffusion method

Franz diffusion cell method

Flow through cell method

in-vitro release method based on in vitro lipolysis model. Lipid nanoparticles are one of rapid developed drug delivery systems in recent years. The indicators to evaluate the lipid nanoparticles are mainly particle size

Zeta potential

drug loadings

in vitro release

stability and so on. Drug release from the carriers is one of the key factors affecting drug performance in vivo

so the in vitro release is the important one to control the quality of the lipid nanoparticles.