HPLC测定醋艾炭间羟基安息香酸的含量

王永丽; 尉小慧; 赵森淼; 刘伟; 李思蒙; 侴桂新; 王峥涛

doi:10.11653/syfj201318078

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HPLC测定醋艾炭间羟基安息香酸的含量

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- HPLC测定醋艾炭间羟基安息香酸的含量
- Content Determination of 3-Hydroxybenzoic Acid in Artemisiae Argyi Folium (Carbonized and Processed with Vinegar) by HPLC
- 中国实验方剂学杂志 2013年19卷第18期页码：78-81
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  国家药品标准提高研究项目(778)
- DOI：10.11653/syfj201318078
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- 纸质出版日期：2013
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王永丽, 尉小慧, 赵森淼, 等. HPLC测定醋艾炭间羟基安息香酸的含量[J]. 中国实验方剂学杂志, 2013,19(18):78-81.

WANG Yong-li, WEI Xiao-hui, ZHAO Sen-miao, et al. Content Determination of 3-Hydroxybenzoic Acid in Artemisiae Argyi Folium (Carbonized and Processed with Vinegar) by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 78-81.
王永丽, 尉小慧, 赵森淼, 等. HPLC测定醋艾炭间羟基安息香酸的含量[J]. 中国实验方剂学杂志, 2013,19(18):78-81. DOI： 10.11653/syfj201318078.

WANG Yong-li, WEI Xiao-hui, ZHAO Sen-miao, et al. Content Determination of 3-Hydroxybenzoic Acid in Artemisiae Argyi Folium (Carbonized and Processed with Vinegar) by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 78-81. DOI： 10.11653/syfj201318078.

摘要

目的: 对不同产地醋艾炭间羟基安息香酸的含量进行比较研究

为醋艾炭的质量控制及合理利用提供科学依据。方法: 采用HPLC

Prevail C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸(15:85)

流速1.0 mL·min-1

检测波长240 nm

柱温25 ℃。结果: 间羟基安息香酸在0.010 02~0.501 00 μg具有良好的线性(r=1.000 0)

平均回收率(n=9) 97.39%

RSD 0.99%。结论: 所建方法准确、简单、重复性好

不同产地醋艾炭间羟基安息香酸的含量差异显著。

Abstract

Objective: To study 3-hydroxybenzoic acid in Artemisiae Argyi Folium (carbonized and processed with vinegar) that come from different arrears

which provide the theoretical basis of the quality control and rational use of Artemisiae Argyi Folium (carbonized and processed with vinegar). Method: HPLC method

the analytical column was Prevail C18 column (4.6 mm×250 mm

5 μm). The mobile phase was acetonitrile-0.1% phosphate (15: 85). The flow rate was 1.0 mL·min-1. The detection wavelength was 240 nm and the column temperature was set at 25 ℃. Result: There was good linearity between the peak areas and concentration at the ranges of 0.010 02-0.501 00 μg for 3-hydroxybenzoic acid (r=1.000 0). The average recovery (n=9) was 97.39% with RSD 0.99%. Conclusion: The method is accurate

simple and repeatable. The 3-hydroxybenzoic acid in Artemisiae Argyi Folium (carbonized and processed with vinegar) of different arrears has significant difference.

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