Objective: To establish an RP-HPLC method for determination of pulsatilla saponin B3 and pulsatilla saponin BD in pulsatilla total saponins emulsions

and determine its in vitro release. Method: Liquid-liquid extraction method was adopted to dispose samples and HPLC was adopted to detect.Chromatographic conditions were as follows:Diamonsil C18 column (4.6 mm×250 mm

5 μm)

C18 guard column (4.0 mm×3.0 mm)

column temperature 40℃

mobile phase of acetonitrile-water-tetrahydrofuran (52:45:3)

flow rate 1.0 mL·min-1

detection wavelength 203 nm

injection volume 10 μL.In vitro release tests of pulsatilla total saponins emulsions and pulsatilla total saponins solution were adopted modified dynamic dialysis. Result: Linear ranges of pulsatilla saponin B3 and pulsatilla saponin BD were 0.01-1(r=0.999 6) and 0.01-2(r=0.999 8) g·L-1

RSD of precision were 3.19% and 1.17%

RSD of repetition were 1.40% and 1.60%

average recoveries were 99.63% and 100.27%.The concentration of pulsatilla saponin B3 in three batches of pulsatilla total saponins emulsions were 0.186

0.190

0.191 g·L-1

the concentration of pulsatilla saponin BD were 0.069

0.065

0.066 g·L-1

respectively.Cumulative release of pulsatilla saponin B3 and pulsatilla saponin BD from emulsions in pH 7.4 phosphate buffer reached 91.30% and 74.97% within 12 h

respectively. Conclusion: This established method was accurate

sensitive and suitable for determination of index components and release of Pulsatillae Radix emulsions.