藤三七总皂苷和总黄酮提取工艺优选

蒋珍藕; 邱宏聪; 邓聿胤

doi:10.11653/syfj2013190028

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藤三七总皂苷和总黄酮提取工艺优选

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- 藤三七总皂苷和总黄酮提取工艺优选
- Optimization of Extraction Technology of Total Saponins and Total Flavonoids from
- 中国实验方剂学杂志 2013年19卷第19期页码：28-30
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西医疗卫生重点科研课题(重2010112)
- DOI：10.11653/syfj2013190028
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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蒋珍藕, 邱宏聪, 邓聿胤. 藤三七总皂苷和总黄酮提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(19):28-30.

JIANG Zhen-ou, QIU Hong-cong, DENG Yu-yin. Optimization of Extraction Technology of Total Saponins and Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 28-30.
蒋珍藕, 邱宏聪, 邓聿胤. 藤三七总皂苷和总黄酮提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(19):28-30. DOI： 10.11653/syfj2013190028.

JIANG Zhen-ou, QIU Hong-cong, DENG Yu-yin. Optimization of Extraction Technology of Total Saponins and Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 28-30. DOI： 10.11653/syfj2013190028.

摘要

目的: 优选藤三七总皂苷和总黄酮的提取工艺。方法: 采用UV测定总皂苷和总黄酮含量。以总皂苷和总黄酮提取量为指标

采用单因素试验考察提取次数

正交试验考察乙醇体积分数、提取时间及乙醇用量对藤三七中总皂苷和总黄酮提取工艺的影响。结果: 藤三七中总皂苷和总黄酮的最佳提取工艺为加12倍量75%乙醇回流提取2次

每次2 h

总皂苷、总黄酮平均提取量分别为16.23

46.40 mg·g-1。结论: 优选的提取工艺合理可行。

Abstract

Objective: To optimize extraction technology of total saponins and total flavonoids from Anredera cordifolia. Method: The contents of total saponins and total flavonoids were determined by UV.With extraction amounts of total saponins and total flavonoids as indexes

extraction times was investigated by single factor test

effects of ethanol concentration

extraction time and ethanol amount on extraction technology of total saponins and total flavonoids were investigated by orthogonal design. Result: The best extraction conditions for total saponins and total flavonoids from A. cordifolia was as following:extracted 2 times with 12-fold the amount of 75% ethanol

2 h per time;Average extraction volume of total saponins and total flavonoids were 16.23

46.40 mg·g-1

respectively. Conclusion: Optimized extraction process was reasonable and feasible.

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