凯氏定氮法和考马斯亮蓝法测定方格 星虫多糖中蛋白质的含量

刘玉明; 钱甜甜; 蒋定文; 何颖; 沈先荣; 江叔奇

doi:10.11653/syfj2013190096

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凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白质的含量

更新时间：2024-01-04

- 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白质的含量
- The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from
- 中国实验方剂学杂志 2013年19卷第19期页码：96-98
- 作者机构：
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- 基金信息：
  
  国家科技重大专项新药创制项目(2008ZXJ09004-027)
- DOI：10.11653/syfj2013190096
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- 纸质出版日期：2013
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刘玉明, 钱甜甜, 蒋定文, 等. 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白质的含量[J]. 中国实验方剂学杂志, 2013,19(19):96-98.

LIU Yu-ming, QIAN Tian-tian, JIANG Ding-wen, et al. The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 96-98.
刘玉明, 钱甜甜, 蒋定文, 等. 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白质的含量[J]. 中国实验方剂学杂志, 2013,19(19):96-98. DOI： 10.11653/syfj2013190096.

LIU Yu-ming, QIAN Tian-tian, JIANG Ding-wen, et al. The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 96-98. DOI： 10.11653/syfj2013190096.

摘要

目的: 建立一种快速、简单、准确测定方格星虫多糖中蛋白质含量的方法。方法: 分别采用凯氏定氮法和考马斯亮蓝法测定蛋白质含量

并进行稳定性、精密度、重复性和回收率测定。结果: 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白的含量分别为2.40%

2.22%

其回收率分别为98.69%

100.97%

2种方法测得蛋白质含量基本相同

无显著性差异(P>0.05)。结论: 综合考虑

凯氏定氮法简便、快速、准确

可用于方格星虫多糖中蛋白质的质量控制。

Abstract

Objective: To establish a simple

rapid and accurate method of protein content analysis of polysaccharide from Sipunculus nudus. Method: Using Kjeldah method and Coomassie brilliant blue method to test protein content of the polysaccharide from S. nudus. Also the precision and accuracy of the two methods were tested. Result: The content of protein was 2.40% and 2.22% by the two method and the average recovery rates were 98.69% and 100.97% respectively. The content by the two methods was almost the same and had no marked difference (P>0.05). Conclusion: In summary

Kjeldah method is simple and accurate and could assay the protein content of polysaccharide from S. nudus rapidly.

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