Objective: To isolate and identify chemical constituents from Phellodendron chinense. Method: Compounds were isolated by silica gel and Sephadex LH-20 column chromatography

and their structures were determined by means of the spectral analysis and physicochemical properties. Result: Eleven compounds were isolated and identified as berberine(1)

obaculactone(2)

shihulimoninA(3)

N-p-coumaroyltyramine(4)

1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxy-propyl)-2-methoxyphenoxy]-propane-1

3-diol(5)

phellodendrine(6)

magnoflorine(7)

palmatine(8)

jatrorrhizine (9)

columbamine (10)

obacunone (11). Conclusion: Compounds 3 and 5 were isolated from P. genus for the first time

and compound 4 was isolated from this plant for the first time.