RP-HPLC测定依折麦布原料药中的有关物质

周增慧; 庄贺飞; 于鑫; 曾颜; 毕开顺; 陈晓辉

doi:10.11653/syfj2013190159

您当前的位置：

首页 >

文章列表页 >

RP-HPLC测定依折麦布原料药中的有关物质

更新时间：2024-01-04

- RP-HPLC测定依折麦布原料药中的有关物质
- Determination of Related Substances in Ezetimibe by RP-HPLC
- 中国实验方剂学杂志 2013年19卷第19期页码：159-163
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013190159
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
周增慧, 庄贺飞, 于鑫, 等. RP-HPLC测定依折麦布原料药中的有关物质[J]. 中国实验方剂学杂志, 2013,19(19):159-163.

ZHOU Zeng-hui, ZHUANG He-fei, YU Xin, et al. Determination of Related Substances in Ezetimibe by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 159-163.
周增慧, 庄贺飞, 于鑫, 等. RP-HPLC测定依折麦布原料药中的有关物质[J]. 中国实验方剂学杂志, 2013,19(19):159-163. DOI： 10.11653/syfj2013190159.

ZHOU Zeng-hui, ZHUANG He-fei, YU Xin, et al. Determination of Related Substances in Ezetimibe by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 159-163. DOI： 10.11653/syfj2013190159.

摘要

目的: 建立测定依折麦布原料药中有关物质的RP-HPLC测定法。方法: Phenomenex Luna C18色谱柱(4.6 mm ×150 mm

5 μm)

流动相0.01%磷酸(A)

乙腈(B)

梯度洗脱

流速1.0 mL·min-1

检测波长231 nm

柱温35℃。结果: 依折麦布与有关物质分离良好;有关物质A

C在0.035~0.8 mg·L-1峰面积与浓度呈良好的线性关系

r分别为0.999 7

0.999 4

0.999 9(n=6)。结论: 方法操作简便

灵敏度高

专属性强

重复性好

可用于依折麦布原料药中有关物质的检查。

Abstract

Objective: To establish a RP-HPLC method for the determination of related substances in ezetimibe. Method: The method was performed on a Phenomenex Luna C18 column (4.6 mm×150 mm

5 μm) using a mobile phase consisting of 0.01% phosphoric acid as the mobile phase A

acetonitrile as the mobile phase B with gradient elution. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 231 nm. The column temperature was set at 35℃. Result: Related substances were completely separated from ezetimibe. The standard curves of substances A

B and C were linear in the range of 0.035-0.8 mg·L-1(r=0.999 7

0.999 4

0.999 9) (n=6)

respectively. Conclusion: The method is simple

sensitive with high specificity and repeatability. It is suitable for the determination of related substances in ezetimibe.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

高效液相色谱法测定轻身减肥片中大黄素与大黄酚的含量

RP-HPLC测定苗药黑白保肝清颗粒中槲皮素的含量