Objective: To investigate distribution characteristics of active ingredients from Scutellariae Radix in heart

liver

stomach

lung

spleen

kidney

and small intestine after rats being intragastric administrated Gegen Qinlian decoction. Method: LC-MS/MS was employed to determine the contents of baicalin

baicalein

wogonoside and wogonin in rat tissue

Shiseido CAPCELL PAK C18 column(2.0 mm×100 mm

5 μm) with a linear gradient system composed of 0.1% formic acid acetonitrile-0.1% formic acid water solution

ESI under positive ion and multi-reaction monitoring mode(MRM) were adopted.Dose of Gegen Qinlian decoction was 15 g·kg-1

2

4

6

8

12 h after administration

rats were sacrificed by breaking arteria carotis

then removed heart

liver

stomach

lung

spleen

kidney and small intestine immediately

distribution of active ingredients in each tissue was determined. Result: Linear range of baicalin

baicalein

wogonoside and wogonin were 6.150-3 077

3.030-1 515

6.170-3 085

3.140-1 569 μg·L-1

RSD of the intra was 5.4%-11.7%

RSD of inter-day precision 7.8%-11.9%

RE of accuracy was-6.4%-7.5%

average recoveries of four active ingredients were more than 75.5%

matrix effects could be ignored

the concentration of four active ingredients in liver tissue were stable under different conditions. Conclusion: Active ingredients concentration was quite different in various tissues

major distribution organs of baicalin

baicalein

wogonoside

wogonin in rats were liver

stomach and small intestine

followed by kidney

heart

lung and spleen.