Objective: To investigate pharmacokinetics process of the major components of Guangtongxiao foam aerosols tetrahydropalmatine (THP) and berberine hydrochloride (Ber) in human. Method: Using LC-MS/MS to measure plasma concentration at different time after rectal administration concentration of Guangtongxiao foam aerosols was determined

Win Nonlin software was used to calculate pharmacokinetic parameters. Result: In the range of 0.000 594-1.188

0.000 536-1.072 μg·L-1

THP

Ber showed good linearity

high

medium and low recovery rates of THP

and Ber

day and inter-day precision met the demands of quantification

Tmax were (132.5±25.5)

(132.5±30.1)min

Cmax were (0.55±0.30)

(0.31±0.32) μg·L-1. Conclusion: LC-MS/MS had good selectivity

high sensitivity for the determination of plasma sample

and can be used successfully in the study of the dynamics of human plasma that contain Guangtongxiao foam aerosols.