Objective: To study the effect of serum containing Gleditsiae Spina on inducing apoptosis and B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax) mRNA expression of human colorectal cancer SW-480 cells. Method: Liquid extracted from Gleditsiae Spina was prepared

the serum containing Gleditsiae Spina was prepared by feeding healthy 50 SD rats at high

medium and low dose of Gleditsiae Spina(2 000

1 000

500 mg·L-1). normal saline and cyclophosphamide (CTX) group(50 mg·kg-1) according to the rat body weight(20 mL·kg-1). The impact of serum containing Gleditsiae Spina on SW-480 with a cell density of 1×106/mL proliferation was estimated by methyl thiazolyl tetrazolium(MTT) at 24

48

72 h after intervened

the apoptosis was detected by flow cytometry(FCM) and Annexinc V-FITCPI. And the mRNA expression variance of the bax and Bcl-2 was used to detect by RT-PCR after intervention with various dose ofserum containing Gleditsiae Spina for 24 h. Result: The serum containing Gleditsiae Spina had obvious inhibition effects on human colorectal cancer SW-480 cells in vitro

the A were significantly lower than that of the blank group(0.370±0.005)%

(0.624±008)%

(1.078±0.038)%(P<0.01)

the apoptosis of human colorectal cancer SW-480 cells (36.918±1.390)%

(33.036±2.972)%

(23.372±1.612)% was significantly lower than that of the blank group(5.036±0.378)% (P<0.01)

the expression of mRNA of Bax(0.258±0.037

0.442±0.024

0.652±0.072)was significantly lower than that of the blank group (0.936±0.047)(P<0.01)

whereas expression of Bcl-2(1.946±0.017

1.810±0.023

1.724±0.028) was higher than that of the blank group(1.260±0.029) (P<0.01). Conclusion: The serum containing Gleditsiae Spina can inhibit proliferation and induce apoptosis of human colorectal cancer SW-480 cells. The decreased expression of Bcl-2 gene and the increased expression of Bax gene may be the mechanism of apoptosis of SW-480.