Objective: To compare the impact of different interventions on the behavior indicators of anxiety rats within the Elevated Plus Maze test(EPM). Method: Sixty SD male rats were randomized to group A (quiet control group

10 rats) and model group(group M0

50 rats). Group M0 received 21d randomal and unpredictable anxiety stimuli for modeling

while at the same time group A received no intervention. Then group M0 was randomly divided into five groups

including model recovery group

Salad oil 4.0 g·kg-1·d-1ig(M1)

model exercise group

Salad oil 4.0 g·kg-1·d-1 ig(M2)

model Rana egg oil group

4.0 g·kg-1·d-1ig(M3)

model exercise-Rana egg oil group

4.0 g·kg-1·d-1 ig(M4)

model drug control group

Diazepam 2.0 g·kg-1·d-1 ig(M5)

10 rats in each group. With different recovery methods each group would have a 28d recovery

and at the end of the recovery

all groups received a EPM (elevated plus maze) experiment

and behavioral indicators were collected to compare the differences. Result: The open arm movement time in group M2

M3 and M4 was (185.3±10.8)

(176.8±10.6)

(177.6±11.1)s

which was significantly higher than that of M1 (145.4±21.6)s. The open arm movement distance (cm) of M2

M3 and M4 was (975.5±27.1)

(945.7±19.7)

(947.1±18.9)cm

which was significantly higher than that of M1 (732.9±18.9)cm; the open arm entries of M2

M3 and M4 were (10.9±2.4)

(11.3±1.5)

(11.6±1.1)

which was significantly higher than that of M1 (7.7±1.5)

(each P<0.05). Conclusion: After a 28 d recovery period

exercise

Rana egg oil

and exercise-Rana egg oil methods could significantly relieve anxiety behavior

the exercise group recovered best

but the natural recovery means had no significant effect after 28 days.