Objective: To investigate effect of serum containing Danggui Buxue decoction on the apoptosis and related protein expression of angiotensin II(Ang II)-induced myocardial. Method: Danggui Buxue decoction was prepared by serum pharmacology method. The cardiomyocytes in the logarithmic growth phase were divided into 4 groups:control group

Ang II(10-7 mol·L-1)group

valsartan(1×10-6mol·L-1) group and 10% medicated serum (0.84 g·L-1)group

each group would be detected after 48 hours. Cell activity was measured by MTT method. Cardiomyocytes DNA damage was detected by Single-cell gel electrophoresis. Laser confocal scanning microscopy was used to detect the mitochondrial membrane potential. Western blotting was used to study the changes of p53 protein and Caspase-3 protein. Result: The myocardial cell activity(A)(0.159±0.024)and mitochondrial membrane potential(Δψm)(170.19±16.15)in Ang II group were significantly lower than those of blank control group(P<0.05)

but myocardial cell DNA damage

p53(0.497±0.029)and Caspase-3(0.509±0.041) protein expression were obviously higher than that of blank control group (P<0.05); the myocardial cell activity(A)(0.197±0.035) and mitochondrial membrane potential(Δψm)(242.39±18.27)in Danggui Buxue decoction containing serum group were significantly higher than those of Ang II group(P<0.05)

but myocardial cell DNA damage

p53(0.387±0.020)and Caspase-3(0.385±0.029)protein expression was obviously lower than that of Ang II group (P<0.05). Conclusion: Danggui Buxue decoction could inhibit the apoptosis of Ang II-induced cardiomyocyte hypertrophy.It may be related with mitochondria-dependent apoptotic pathway.