Objective: To study effect of platelet rich plasma combined with bone marrow mesenchymal stem cells at different consistency on rabbit cartilage defect. Method: Sixty rabbits were randomly divided into control group

platelet-rich plasma(PRP) group(3%)

bone mesenehymal stem cells(BMSCs) group(1×106/mL)and combination group(PRP+BMSCs 1:5

2:5

3:5).The models of rabbit cartilage defect were made by operation.Then the models were injected combination at different consistency once a week from 2th week to 5th week after operation. The rabbit cartilage defects were observed by Masson trichrome coloration. Semi-quantitative scoring of articular cartilage were computed by Pineada. Repair of articular cartilage defect in rabbits were observed. Result: Compared with the control group

semi-quantitative scoring of articular cartilage in each combination group decreased(P<0.05). In combination groups

semi-quantitative scoring of articular cartilage was lower when PRP at higher consistency. PRP combined with BMSCs(3:5) was lowest in combination groups(P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration and improve bone tissue recovery. The recovery of articular cartilage defects was related with PRP concentration.