Objective: To observe the protective effect of Jidesheng Sheyao

a Chinese patent medicine

on liver fibrosis of mouse caused by carbon tetrachloride(CCl4) and to explore the action mechanism of the drug. Method: Forty-eight ICR mice were randomly divided into four groups:the normal control group

the model group

treatment group 1 and treatment group 2.All mice were subcutaneously injected with 40%CCl4 for 5 weeks in order to prepare liver fibrosis model. Meanwhile

the mice of two treatment groups were given with Jidesheng Sheyao in different doses and the normal the mice of control group were given with distilled water in the same volume

by intragastric administration. The concentrations of hyaluronic acid (HA) and laminin (LN) in serum of mouse were measured with the method of radioimmunoassay. Masson trichrome staining was adopted to prepare hepatic tissue section. The pathological variations in hepatic tissue were observed under light microscope and the score of hepatic fibrosis was evaluated. The rabbits were given with Jidesheng Sheyao by intragastric administration to prepare medicated serum. The hepatic stellate cell strain (HSC-T6) was taken as the model for study in vitro. HSC cells were cultured with medicated serum in different concentrations. The proliferation of HSC was assessed with CCK-8 assay. Labeled with annexin V/PI

the apoptosis of HSC was analyzed with flow cytometry. Result: Compare with mice of model group

the severities of fibrosis in mice in treatment groups were obviously alleviative

the levels of fibrosis score and the serum content of HA

LN of mice in treatment groups were all lower. After cultured with the medicated serum respectively in the concentration of 10% and 12%

the proliferations of HSC were obviously inhibited and the apoptosis rates of the cells were significantly increased

compared with the cells of control group cultured with normal rabbit serum. Conclusion: Jidesheng Sheyao can inhibit the proliferation of HSC and promote the apoptosis of HSC

so the drug has some efficacy of anti-hepatic fibrosis.