Objective: To study the effect

of big leaf Gentianae Macrophyllae Radix alcohol extract on serum anti-cyclic citrullinafer peptide(CCP)and tumor necrosis factor-α(TNF-α) in collagen induced arthritis rat control. Method: Ninty-six rats were randomLy divided into:normal group

model group

Paeoniae Alba Radix and Gentianae Macrophyllae Radix group group. In addition to the normal group

each group was given collagen emulsion multipoint to establish arthritis model. After modelling

corresponding drugs was given on day 14

21 and 28

the general status

and joint swelling degree were observed. ELISA method was used to detect serum anti-CCP antibody and TNF-α level. Result: In model rats

ankle and digits joint swelling was obvious and serum anti-CCP antibody and TNF-α level obviously increased

in Gentianae Macrophyllae Radix group and radix paeoniae alba group

compared with model group ankle joint swelling and toes significantly reduced and serum anti-CCP antibody and TNF-α level decreased obviously

there was a statistical significance (P<0.05);after 28 days in Gentianae Macrophyllae Radix group compared with Paeoniae Alba Radix group

joint swelling of rats significantly reduced and TNF-α reduced obviously (P<0.05). Conclusion: The big leaf Gentianae Macrophyllae Radix alcohol extract can decrease in collagen induced arthritis rats serum anti-CCP antibody and TNF-α level

reduce joint swelling degree

and improve the synovial inflammation shape.