Objective: To prepare limonin creams and investigate its quality stability. Method: Creams was prepared with limonin as raw material

HPLC was adopted to determined the content and related substances of limonin

chromatographic conditions were as follows:Zorbax SB-C18 column(4.6 mm×150 mm

5 μm)

Zorbax SB-C18 guard column(4.6 mm×12.5 mm

5 μm)

mobile phase of water-acetonitrile(42:58)

detection wavelength 207 nm

flow rate 1.0 mL·min-1

column temperature at room temperature

injection volume 20 μL

stability of three batches of limonin creams were carried out. Result: Limonin creams showed milky white

its identification and inspection items were in line with relevant regulations

the linear range of limonin was 20.26-101.3 mg·L-1 with average recovery of 99.6%(RSD 0.68%).The mass fraction of limonin in three creams samples was 100.4%-101.6%

quality of samples was stable in different environments except in high humidity or high temperature

where volume would be slightly increased or decreased. Conclusion: This preparation technology was simple and controllable

it should be saved in cool and dry place.