Objective: To optimize ultrasonic-assisted enzymatic hydrolysis technology of baohuosideⅠ. Method: The content of baohuosideⅠ was determined by HPLC

chromatographic conditions were as follows:Diamond C18 column(4.60 mm×250 mm

5 μm)

mobile phase of acetonitrile-water(25:75)

flow rate 1.0 mL·min-1

column temperature 30℃

detection wavelength 270 nm.Taking bioconversion rate of baohuosideⅠ as index

based on single factor tests

effects of ultrasonic power

pH

temperature

enzyme dosage and reaction time on preparation technology were investigated L9(34) orthogonal design. Result: Optimal enzymatic conditions were:ultrasonic power of 200 W

pH of 5.0

reaction temperature at 40℃

reaction time of 4 h

enzyme dosage 5%.Under these conditions

conversion rate of baohuosideⅠ was 97.4%. Conclusion: Ultrasonic-assisted enzymatic method could improve conversion rate of baohuosideⅠ with low reaction temperature

short reaction time

little enzyme and other advantages

optimized process was stable

simple and suitable for industrial production.