Objective: To investigate effect of pretreatment methods on the content of cholic acid from sheep bile. Method: Cholic acid was prepared by direct drying method

saponification method

vacuum freeze drying method

centrifugation method

respectively.The content of cholic acid was determined by UV

amd effect of pretreatment methods on the content of cholic acid from sheep bile was investigated. Result: Effect of different processing methods on the content of cholic acid was great

contents of cholic acid from saponification crude products

saponification refined products

freeze-dried products

centrifugation drying products1

centrifugation drying products2 and gallbladder direct drying products were 63.41%

79.55%

39.67%

30.62%

41.71%

36.26%

respectively. Conclusion: Comprehensive evaluation with yield of dry products and the content of cholic acid

direct centrifugation combining with adding 95% ethanol removal of protein composition and vacuum freeze drying method were better than other methods.