HPLC测定密蒙花配方颗粒中蒙花苷含量

唐维宏; 韦建华; 马云婷

doi:10.11653/syfj2013200050

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HPLC测定密蒙花配方颗粒中蒙花苷含量

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- HPLC测定密蒙花配方颗粒中蒙花苷含量
- Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC
- 中国实验方剂学杂志 2013年19卷第20期页码：50-51
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- DOI：10.11653/syfj2013200050
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- 纸质出版日期：2013
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唐维宏, 韦建华, 马云婷. HPLC测定密蒙花配方颗粒中蒙花苷含量[J]. 中国实验方剂学杂志, 2013,19(20):50-51.

TANG Wei-hong, WEI Jian-hua, MA Yun-ting. Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 50-51.
唐维宏, 韦建华, 马云婷. HPLC测定密蒙花配方颗粒中蒙花苷含量[J]. 中国实验方剂学杂志, 2013,19(20):50-51. DOI： 10.11653/syfj2013200050.

TANG Wei-hong, WEI Jian-hua, MA Yun-ting. Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 50-51. DOI： 10.11653/syfj2013200050.

摘要

目的: 建立密蒙花配方颗粒中蒙花苷的含量测定方法。方法: 采用Inertsil ODS3 C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%磷酸(55:45)

流速1.0 mL·min-1

柱温20℃

检测波长327 nm

进样量10 μL。结果: 蒙花苷进样量在0.104 8~1.048 μg呈良好线性关系(r=0.999 6)

平均加样回收率102.2%(RSD 1.9%)。结论: 该方法操作简便、结果准确、重复性良好

可作为密蒙花配方颗粒中蒙花苷的含量测定方法。

Abstract

Objective: To establish a method for determining buddleoside in Buddlejae Flos formula granules. Method: Buddleoside was separated on Inertsil ODS3C18 column(4.6 mm×250 mm

5 μm) with methanol-0.1% phosphoric acid(55:45) as mobile phase

flow rate was 1.0 mL·min-1

column temperature was at 20℃

detection wavelength was 327 nm

injection volume was 10 μL. Result: Buddleoside had good linear relationship within the range of 0.104 8-1.048 μg with r=0.999 6

the average recovery was 102.2% with RSD 1.9%. Conclusion: This method was simple

accurate and reproducible

it could be used to determine the content of buddleoside in Buddlejae Flos formula granules.

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