雷公藤多苷中雷公藤对醌B的LC-MS定性和HPLC定量分析

付辉政; 钟瑞建; 罗跃华; 王栋; 周国平; 张东明; 万凯化

doi:10.11653/syfj2013200084

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雷公藤多苷中雷公藤对醌B的LC-MS定性和HPLC定量分析

更新时间：2024-01-04

- 雷公藤多苷中雷公藤对醌B的LC-MS定性和HPLC定量分析
- Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC
- 中国实验方剂学杂志 2013年19卷第20期页码：84-87
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  国家药典委员会项目(TS-33)
- DOI：10.11653/syfj2013200084
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- 纸质出版日期：2013
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付辉政, 钟瑞建, 罗跃华, 等. 雷公藤多苷中雷公藤对醌B的LC-MS定性和HPLC定量分析[J]. 中国实验方剂学杂志, 2013,19(20):84-87.

FU Hui-zheng, ZHONG Rui-jian, LUO Yue-hua, et al. Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 84-87.
付辉政, 钟瑞建, 罗跃华, 等. 雷公藤多苷中雷公藤对醌B的LC-MS定性和HPLC定量分析[J]. 中国实验方剂学杂志, 2013,19(20):84-87. DOI： 10.11653/syfj2013200084.

FU Hui-zheng, ZHONG Rui-jian, LUO Yue-hua, et al. Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 84-87. DOI： 10.11653/syfj2013200084.

摘要

目的: 建立雷公藤多苷中雷公藤对醌B定性分析和含量测定方法。方法: 利用高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)联用结合LC-DAD技术

通过和标准化合物雷公藤对醌B的保留时间、紫外吸收光谱以及质谱中的准分子离子峰[M+Na]+相对照

快速鉴定雷公藤多苷中的雷公藤对醌B

对其含量的测定则采用了高效液相色谱方法。结果: 一级质谱扫描结果表明雷公藤多苷中保留时间tR=13.2 min的峰化合物结构为雷公藤对醌B

准分子离子峰[M+Na]+为353.1

高效液相色谱结果表明雷公藤多苷中雷公藤对醌B在0.115~1.15 μg呈良好的线性关系(r=0.999 9)

平均回收率和RSD分别为99.32%

0.7%。结论: 该方法简便、准确

可作为雷公藤多苷中雷公藤对醌B的含量测定方法。

Abstract

Objective: To develop an analytic method for determination of triptoquinone B in tripterygium glycosides. Method: The HPLC-ESI-MS and HPLC-DAD methods were established. Triptoquinone B in tripterygium glycosides was quickly identified by comparing the retention time

UV absorption spectrum and quasi-molecular ion peak [M+Na]+ with the reference compound. The content of triptoquinone B in tripterygium glycosides was determined by HPLC. Result: One level mass spectrometry scanning results showed that the chromatographic peak of retention time tR=13.2 min was identified as triptoquinone B

and its quasi-molecular ion peak [M+Na]+ was 353.1.The calibration curves of triptoquinone B was linear between 0.115-1.15 μg (r=0.999 9). The average recovery and the relative standard deviation were 99.32% and 0.7% respectively. Conclusion: The method is simple

accurate and could be used for determination of triptoquinone B in tripterygium glycosides.

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