UPLC同时测定野菊花中蒙花苷和绿原酸含量

韩永成; 龚海燕; 刘伟; 陈宁; 崔永霞; 吴明侠; 王丽果; 茹凡书

doi:10.11653/syfj2013200088

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UPLC同时测定野菊花中蒙花苷和绿原酸含量

更新时间：2024-01-04

- UPLC同时测定野菊花中蒙花苷和绿原酸含量
- Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC
- 中国实验方剂学杂志 2013年19卷第20期页码：88-90
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省教育厅自然科学研究计划项目 (2010A360016)
- DOI：10.11653/syfj2013200088
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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韩永成, 龚海燕, 刘伟, 等. UPLC同时测定野菊花中蒙花苷和绿原酸含量[J]. 中国实验方剂学杂志, 2013,19(20):88-90.

HAN Yong-cheng, GONG Hai-yan, LIU Wei, et al. Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 88-90.
韩永成, 龚海燕, 刘伟, 等. UPLC同时测定野菊花中蒙花苷和绿原酸含量[J]. 中国实验方剂学杂志, 2013,19(20):88-90. DOI： 10.11653/syfj2013200088.

HAN Yong-cheng, GONG Hai-yan, LIU Wei, et al. Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 88-90. DOI： 10.11653/syfj2013200088.

摘要

目的: 建立UPLC同时测定不同产地野菊花中蒙花苷和绿原酸含量的分析方法。方法: 采用超高效液相色谱系统

C18 色谱柱(2.1 mm×50 mm

1.8 μm)

流动相乙腈-0.1%磷酸水

以0.2 mL·min-1的流速进行梯度洗脱

检测波长326 nm

柱温25℃。结果: 蒙花苷在3.0~120.0 mg·L-1线性关系良好(r=1.000 0)

平均回收率99.43%

RSD 1.54%(n=6);绿原酸在0.48~19.2 mg·L-1线性关系良好(r=1.000 0)

平均回收率98.67%

RSD 2.13%(n=6)。结论: UPLC法分析速度快

重复性好

结果准确且高效、简便

可用于野菊花中蒙花苷和绿原酸含量的测定。

Abstract

Objective: To establish a UPLC method for simultaneous determination of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos from different areas. Method: UPLC was used. The chromatographic separation was performed on a C18 (2.1 mm×50 mm

1.8 μm) column with gradient elute. The flow rate was 0.2 mL·min-1. The detection wavelength was set at 326 nm and column temperature was maintained at 25℃. Result: Good linear relationship between the peak area and content of buddleoside and chlorogenic acid was achieved when the amount was within 3.0-120.0

0.48-19.20 mg·L-1;the recoveries were 99.43% and 98.67%;RSDs were 1.54% and 2.13% respectively(n=6). Conclusion: The UPLC analysis is speed

reproducible

accurate

efficient and convenient

which can detect the content of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos.

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